Difference between revisions of "Bradford Assay"
From Bridges Lab Protocols
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##Set dilution to be 1/vol (ie 0.1 for 10 uL) | ##Set dilution to be 1/vol (ie 0.1 for 10 uL) | ||
##Blank then measure samples, absorbance must be less than 0.9 | ##Blank then measure samples, absorbance must be less than 0.9 | ||
| − | ##Print and attach to experiment | + | ##Print (hit Recall, then enter, then print) and attach to experiment |
==Reference== | ==Reference== | ||
Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]] | Wikipedia: [[wikipedia:Bradford_protein_assay|Bradford Protein Assay]] | ||
<pubmed>942051</pubmed> | <pubmed>942051</pubmed> | ||
Revision as of 15:51, 8 July 2009
Materials
- BioRad Protein Assay Dye Reagent Concentrate cat#500-0006
- Disposable Plastic Cuvette
Protocol
- Dilute reagent 5X in water, stable for 2-3 weeks
- Pipet 1 mL into disposable plastic cuvette
- Add 1-10 uL of protein sample, cover with parafilm and mix
- Let sit 5-10 min to react
- Set spectrophotometer as follows:
- Go to protein assay then Bradford assay
- Set formula, then select more
- Set b=0.045 (or determine slope)
- Set dilution to be 1/vol (ie 0.1 for 10 uL)
- Blank then measure samples, absorbance must be less than 0.9
- Print (hit Recall, then enter, then print) and attach to experiment
Reference
Wikipedia: Bradford Protein Assay <pubmed>942051</pubmed>
