View source for Amylose pull-down of glycogen-bound proteins

==Protocol==
* All steps on ice/cold room
* Add 50 ul beads (amylose resin #E80021L Biolabs) to 0.5 mg of protein lysate in lysis buffer with protease inhibitors
* Add 1 ml of lysis buffer
* Rotate in cold room 30’ to ON
* Spin down at max speed for 1’
* Aspirate supernatant down to 1 mm over beads with crushed tip.
* Repeat 3-5 washes with lysis buffer without protease inhibitors 
* After last wash and aspiration spin again and aspirate all supernatant
* Ad 50ul SDS loading buffer, boil for 5 minutes
* Load 10ul/gel