View source for 4EBP1/2 Genotyping

==PRIMERS==
'''4EBP1'''
*4EBP1- Fwd: GAT GGA GTG TCG GAA CTC ACC (1X)
*4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X)
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)

WT= 175bp  KO= 550bp

'''4EBP2'''
*4EBP2-Fwd: GGT GGG ACT GTC GGT CTT CTG (1X)
*4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X)
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)

WT= 450bp  KO= 750bp

Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately.

[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
[[ Category:PCR]]
[[ Category:DNA]]
[[ Category:Molecular Biology]]
[[ Category:mTORC1 ]]