RIPA Buffer
From Bridges Lab Protocols
RIPA Buffer (for 10mL lysis buffer)
Final Concentration | per 10 mL | Stock | Location | |
---|---|---|---|---|
Tris pH7.4 | 50mM | 500uL | 1M | Chemical Shelf |
Na Deoxycholate | 0.25% | 250uL | 10% | Chemical Shelf |
NP-40 | 1% | 1mL | 10% | Chemical Shelf |
NaCl | 150mM | 375uL | 4M | Chemical Shelf |
EDTA | 1mM | 20uL | 0.5M | Chemical Shelf |
NaVO3 (see preparation ) | 100uM | 10uL | 100mM | Chemical Shelf |
NaF | 5mM | 100uL | 500mM | Chemical Shelf |
NaPPi | 10 mM | 1 mL | 100mM | Chemical Shelf |
Protease Inhibitors | 1 mini tablet | 4C |