TC10b Genotyping

Primers

• TC10b - PDFor1: GGAGTCTATGGACATGAGAG
• TC10b - PD4-43: GATCATGACTAGCATGGAGTAG
• TC10b - PDHomoFB: GTCTATGGACATGAGAGTTTC
• TC10b - NEO3A: GCAGCGCATCGCCTTCTATC

Genotyping Program

• 94 2min
• 50 cycles of:
  • 94 30s
  • 56 30s
  • 72 4min
• 72 7min
• 4 forever

Results

WT allele ~ 1kb, KO allele ~ 500bp HET should get two bands