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- [[ Category: Protein-Lipid Interactions ]] ...1 KB (184 words) - 20:55, 31 January 2011
- [[Category:Protein]] ...882 bytes (140 words) - 21:43, 5 January 2018
- #Inject protein samples adjusting contact time as necessary to reach saturation. Typically [[Category: Protein-Lipid Interactions]] ...5 KB (753 words) - 14:40, 14 April 2011
- #Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]]) ...2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer ...1 KB (253 words) - 16:06, 25 April 2019
- * Protein of interest to be immobilized. Ideally start with 10-100 ug/mL protein (need ~100 uL per surface) ...to start). Lower pH will give better immobilization, but potentially less protein function ...2 KB (302 words) - 14:43, 16 June 2010
Page text matches
- ...n Tris, cannot have any free primary amines other than protein, we dialyze protein into PBS before labelling) *Add 350uL of PBS and 25ug of protein to an eppie. ...1,023 bytes (170 words) - 16:27, 27 October 2009
- #Calculate extinction coefficient for protein of interest ...] tool to calculate the extinction coefficient in both molar and mg/mL for protein. Assume all Cys residues are half-cysteines. ...702 bytes (104 words) - 14:03, 11 May 2009
- *BioRad Protein Assay Dye Reagent Concentrate cat#500-0006 #Add 1-10 uL of protein sample, cover with parafilm and mix ...1 KB (202 words) - 14:39, 15 March 2018
- ! Protein [[Category:Protein Purification]] ...444 bytes (57 words) - 17:54, 24 July 2012
- * combine 0.5 ml lysate, 0.2-0.5 ug of antibody and 25 ul of protein A/G plus agarose beads (catalog # sc 2003 santa cruz). * for tissue lysates - use 0.5 mg of protein, add primary antibody first for 0.5 hour and then add beads. ...1 KB (237 words) - 14:23, 21 August 2016
- *Express and induce protein in culture under appropriate conditions: ...ncentration of IPTG and duration of induction should be optimized for each protein) ...2 KB (337 words) - 14:40, 12 August 2009
- *Express and induce protein in culture under appropriate conditions: ...ncentration of IPTG and duration of induction should be optimized for each protein) ...2 KB (340 words) - 22:00, 2 December 2010
- Useful for blocking protein synthesis ...156 bytes (23 words) - 12:55, 11 July 2014
- [[ Category: Protein Purification ]] [[ Category: Protein-Protein Interactions ]] ...3 KB (572 words) - 17:28, 31 July 2012
- *Protein of interest (free of DTT/Glycerol/Glutathione) #Prepare assay volumes of 50 uL in TLA-100 tubes with ~10 uM protein and varying lipid concentrations (for a first try use 0-1 mM lipid at 10X d ...2 KB (260 words) - 13:01, 2 November 2010
- #Check that the HPLC is set up for protein work and not radioactive work (see [[HPLC - Plumbing Setup]]). ##Place the protein lead into the equillibration buffer and prime. ...3 KB (559 words) - 19:47, 9 February 2011
- #Follow Protein Lysate instructions for Bradford Assay (see [[Bradford Assay]]) ...2X loading buffer with B-ME to each lyaste. This will generate a 2 mg/mL protein solution in SDS Loading Buffer ...1 KB (253 words) - 16:06, 25 April 2019
- * Protein of interest to be immobilized. Ideally start with 10-100 ug/mL protein (need ~100 uL per surface) ...to start). Lower pH will give better immobilization, but potentially less protein function ...2 KB (302 words) - 14:43, 16 June 2010
- ===Protein Analysis=== *[[Surface Plasmon Resonance - Protein Lipid Interactions]] ...4 KB (484 words) - 12:10, 15 August 2016
- ...e first Refseq mRNA (should start with NM) then click on that and find the protein (should start with NP) ...gov/Blast.cgi?PROGRAM=blastp&PAGE_TYPE=BlastSearch&LINK_LOC=blasthome NCBI Protein Blast]. ...3 KB (360 words) - 13:16, 18 April 2019
- *Express and induce protein in culture under appropriate conditions: ...ncentration of IPTG and duration of induction should be optimized for each protein) ...3 KB (440 words) - 17:55, 24 July 2012
- Protein must be extensively dialysed in PBS to remove any amine group from the samp #Dissolve protein ligand (~1mg) in 6 mL of coupling buffer. ...2 KB (409 words) - 00:15, 27 May 2009
- [[ Category: Protein Purification ]] [[ Category: Protein-Protein Interactions ]] ...2 KB (355 words) - 17:46, 31 July 2012
- *Protein Markers, we use SeaBlue Plus2 from Invitrogen (cat #LC5925) ...comb out and rinse with water. Pick a percent gel based on your expected protein size. ...3 KB (477 words) - 17:46, 20 January 2025
- # Measure protein content by bradford assay for normalization ...ter glycosidase treatment and are presented in equivalent glucose units/mg protein. ...1 KB (173 words) - 18:23, 30 June 2022