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• Purification of miRNA and mRNA with TRIzol
  ...k RNA prep kit is likely to lose much of the miRNAs. Ideally the PureLink mRNA or miRNA kit would be used. This protocol is largely taken from the TRIzol
  2 KB (280 words) - 21:35, 29 December 2014

Page text matches

• Antisense Oligo Knockdown in Mice
  ...designed siRNA sequences make sure sequences are antisense! (direction on mRNA in blast should be from larger to smaller position). Regular siRNAs are dup
  2 KB (373 words) - 20:43, 2 August 2011
• First Strand cDNA Synthesis (AB Kit)
  [[Category:mRNA]]
  1 KB (154 words) - 15:16, 24 July 2019
• Primer Design for qPCR
  ....ncbi.nlm.nih.gov/gene (make sure its the correct species). Then pick the mRNA you want to probe, based on the isoform structure on that page. Click on t # If you want to look at a specific region of the mRNA and an appropriate primer pair in that region is not selected, change the r
  1 KB (249 words) - 17:48, 29 June 2017
• Designing and Generating CRISPR-Cas Mutants
  ...f interest spans several introns, you need to copy the genomic DNA not the mRNA sequence. In other words, if you can design your CRISPR pairs to be within ...in the CRISPR tool output). Use the actual nucleotide from the reference mRNA sequence, not the nucleotide based on the arbitrary region that you pasted
  2 KB (417 words) - 12:48, 14 July 2015
• Purification of miRNA and mRNA with TRIzol
  ...k RNA prep kit is likely to lose much of the miRNAs. Ideally the PureLink mRNA or miRNA kit would be used. This protocol is largely taken from the TRIzol
  2 KB (280 words) - 21:35, 29 December 2014
• TaqMan miRNA Assay
  * miRNA template, purified via miRNA kit or from [[ Purification of miRNA and mRNA with TRIzol‎ ]]. Need 1-10 ng per reaction The general outline is to make a probe-specific cDNA (as opposed to mRNA qPCR where the same cDNA is used for several probes) then to quantify this
  1 KB (234 words) - 18:52, 30 December 2014
• Quantification of miRNA by SYBR Green qPCR
  * Purify total RNA, via the protocol: [[ Purification of miRNA and mRNA with TRIzol ]] * Can use a protocol similar to the [[ QPCR ]] for mRNA quantification
  3 KB (432 words) - 15:24, 1 March 2017
• Sequence Alignment for Phylogenetic Analysis
  * To find a seed sequence start with NCBI Gene, then find the first Refseq mRNA (should start with NM) then click on that and find the protein (should star ...[https://www.ncbi.nlm.nih.gov/refseq/ RefSeq mRNA]. If there are multiple mRNA's for the gene, name them
  3 KB (360 words) - 13:16, 18 April 2019