#Rehydrate in HBS to a concentration of 25 mM (total lipid)
...10 uM protein and varying lipid concentrations (for a first try use 0-1 mM lipid at 10X dilutions)
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2 KB (260 words) - 13:01, 2 November 2010
#Combine DOPC + C16 lipid (or DOPC alone) at a ratio of 97:3 and dry under nitrogen (30 uL PC or 29.1
#Resuspend to 10 mM total lipid with HBS-N (300 uL). Vortex thoroughly and sonicate in water bath
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5 KB (753 words) - 14:40, 14 April 2011
*Lipid Extraction Mix (10 mL Butanol, 2 mL Ethyl Ether and 0.5 mL Ethyl Formate)
##Remove supernatant to tube with 0.5 mL Lipid Extraction Mix
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2 KB (238 words) - 15:44, 7 August 2009
*[[Inositol Labeling and Lipid Extraction]]
===Protein Analysis===
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4 KB (484 words) - 12:10, 15 August 2016
[[Category: Lipid Analysis]]
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8 KB (1,193 words) - 13:28, 21 June 2010