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- 1 KB (162 words) - 16:57, 8 June 2020
- 1 KB (161 words) - 17:58, 5 June 2009
- # Tail digest DNA *[[PCR Amplification of DNA]] ...773 bytes (111 words) - 17:00, 8 June 2020
- #Resuspend DNA in 6 mL of distilled water. Add 10g of CsCl and dissolve. ...1 KB (167 words) - 22:03, 17 January 2012
- # Select '''Enter Individual DNA Sequencing Requests''' * 1 uL of template (unless the plasmid DNA concentration is >500 ng/uL in which case dilute it to 50-300 ng/uL ...646 bytes (108 words) - 18:34, 5 March 2014
- This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been comp ...sion or use the shaker. '''NEVER''' vortex samples, as it will destroy the DNA. ...3 KB (417 words) - 17:42, 25 September 2015
- 1 KB (179 words) - 21:24, 3 November 2009
- [[Category:DNA]] ===Yeast DNA Prep Buffer=== ...1 KB (197 words) - 16:32, 21 February 2012
- [[Category:DNA]] ...e Yeast. See [[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction. ...1 KB (248 words) - 16:39, 21 February 2012
- ===DNA Extraction=== ** 1 uL of the Extracted DNA from the previous step. ...2 KB (262 words) - 20:02, 13 July 2015
- 6 KB (867 words) - 19:50, 13 September 2019
Page text matches
- *Calculate DNA to transfect. Start with 4 ug per 6 well *Lipofectamine(in uL) = DNA(in ug) * 2.5 ...1 KB (196 words) - 16:57, 22 February 2013
- *Calculate DNA to transfect *Lipofectamine(in uL) = DNA(in ug) * 2.5 ...1 KB (155 words) - 21:00, 11 July 2012
- * DNA - typically transfect 50-1000 ng DNA per well ##Per ug of DNA need 3 uL Fugene. ...1 KB (198 words) - 13:22, 29 August 2011
- * D-Loop Primers (for determining the number of mitochondrial DNA copies) * DNA Primers (for determining the number of nuclear DNA copies). Can use any genotyping primers with an amplicon <200bp. We use t ...1 KB (201 words) - 17:27, 4 April 2023
- # Tail digest DNA *[[PCR Amplification of DNA]] ...773 bytes (111 words) - 17:00, 8 June 2020
- ===DNA Extraction=== ** 1 uL of the Extracted DNA from the previous step. ...2 KB (262 words) - 20:02, 13 July 2015
- *OptiMEM(in uL, equal for DNA and Lipofectamine) = Lipofectamine(in uL) * 50 ##Combine the two volumes of OptiMEM/DNA and OptiMEM/Lipofectamine (should be 200uL/well). ...1 KB (148 words) - 16:39, 18 December 2009
- # Select '''Enter Individual DNA Sequencing Requests''' * 1 uL of template (unless the plasmid DNA concentration is >500 ng/uL in which case dilute it to 50-300 ng/uL ...646 bytes (108 words) - 18:34, 5 March 2014
- *DNA of interest (1 uL for plasmid, 5 uL for cloning/mutagenesis) #Add DNA to cells and mix by tapping ...598 bytes (104 words) - 15:44, 12 February 2014
- [[Category:DNA]] ...e Yeast. See [[Rapid Isolation of Genomic DNA from Yeast]]. Use 1µl of the DNA in your PCR reaction. ...1 KB (248 words) - 16:39, 21 February 2012
- [[ Category:DNA]] ...264 bytes (33 words) - 15:14, 5 June 2020
- * Prepare DNA in a sterile 1.5 mL tube or tubes as needed. Use 100 uL per well. * Add 3 uL PEI per ug of DNA in OptiMEM. The total volume should be 100 uL per well. if transfecting m ...1 KB (199 words) - 16:15, 19 December 2017
- ...our region of interest spans several introns, you need to copy the genomic DNA not the mRNA sequence. In other words, if you can design your CRISPR pairs * Based on where you want to cut the DNA select the one or two guide DNA sequences. Name those sequences based on the nucleotide it cuts after (see ...2 KB (417 words) - 12:48, 14 July 2015
- *GST-HA-S6K1 plasmid. Prepare by [[Cesium Chloride Preparation of DNA]]. Need 500 ug per 10 plates of cells #Combine 500 ug DNA with 10 mL OptiMEM, and separately 700 uL Lipofectamine 2000 with 10 mL Opt ...1 KB (185 words) - 19:59, 20 September 2012
- This protocol is for extracting DNA from TRIZOL preparations after the RNA-containing upper phase has been comp ...sion or use the shaker. '''NEVER''' vortex samples, as it will destroy the DNA. ...3 KB (417 words) - 17:42, 25 September 2015
- [[ Category:DNA]] ...301 bytes (43 words) - 18:42, 25 April 2018
- [[ Category:DNA]] ...e targeted allele only. For Southern blotting, SalI/EcoRI-digested genomic DNA was probed with a 0.4-kb fragment immediately upstream of the 5′ arm (Suppl ...2 KB (225 words) - 15:14, 6 August 2012
- *Sample Genomic DNA (extracted as described in [[Blood DNA Extraction]]) ...1 KB (155 words) - 16:39, 16 November 2015
- * Prepare DNA according to this table in a sterile 2 mL tube: * For this amount of DNA (28 ug) try using 3:1 PEI:DNA, so add 84 uL PEI into 1 mL OptiMEM. ...2 KB (329 words) - 16:20, 8 December 2017
- [[ Category:DNA]] ...495 bytes (59 words) - 14:55, 5 July 2013