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==Materials==
*FBS Media (L1-FBS for 3T3-L1, COS-FBS for others): Prepare by filtering together 5 mL PSG, 50 mL COS-FBS and one bottle of DMEM
*PBS -/-
*0.05% Trypsin
==Protocol==
#Warm PBS and Mediain water bath
#Wash cells twice with 10 mL (per 10 cm dish) PBS -/-
#Add 1 mL trypsin and sit in the hood
