34
edits
Changes
m
→Protocol: Changed wash times
#Block with 2% BSA in [[ TBST ]] or 5% skim milk powder in TBST for >1h
#Incubate with primary antibody (check for dilution) in 1 mg/mL BSA for >1h
#Wash blot every five 5 minutes for 30 15 min with TBST.
#Incubate with appropriate secondary antibody (10 000X) for 45min-1h
#Wash blot every five 5 minutes for 30 15 min with TBST.
#Rinse once or twice with double distilled water
#Prepare ECL by mixing one volume of the black bottle solution with one volume of the white bottle solution (be careful not to cross-contaminate ECL bottles with wrong solution).
