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GST-EEA1 Pulldown from Yeast

20 bytes added, 17:48, 31 July 2012
added link to 2xHNG
 
== '''Materials''' ==
*[[ 2xHNG_Buffer | 2x HNG ]] (100mL). Combine 100mM HEPES (10mL), 300mM NaCl (7.5mL), 20% glycerol (20mL)
*1x HNG (10mL). Mix 5mL 2xHNG and 1 PI tablet.
*Lysis Buffer (10mL). Combine 5mL 1x HNG, 1 PI tablet, 1mL of 1% NP40, and 50µL of magnesium chloride.
Sheelak
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Retrieved from "http://bridgeslab.sph.umich.edu/protocols/index.php/Special:MobileDiff/691"

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