915
edits
Changes
added ethanol to materials and prepared TRIzol tubes first
*Chloroform (in solvent cabinet)
*Label tubes, for each sample need 3 eppies, at least one of which is 2 mL and one of which is a recovery tube from the PureLink kit.
*70% Ethanol make with RNAase free water and 100% Ethanol.
==Protocol==
#Add 1 mL TRIzol reagent to each 2 mL tube.#Cut ~50-100 mg of tissue. If tissue is in RNAlater, cut at room temperature. If tissue is frozen cut on dry ice. Weigh into a fresh 2mL eppendorf tube and keep on dry ice (if frozen).#Add 1 mL TRIzol reagent to each tube.
#Using tissue grinder, homogenize tissue for ~30s until homogeneous (no clumps).
#Incubate 5 minutes at room temperature.
