Changes

Real Time PCR From Cell Culture

107 bytes added, 00:42, 29 March 2011

added reference for calculating amounts from Ct

#Using a multichannel pipettor, add 10 uL Master mix to each well. If using a 384 well plate use 5uL

#Start qPCR Machine using the machine in the [[qPCR - Lin Lab|Lin Lab]] or the [[qPRC - Saltiel Lab|Saltiel Lab]] machine

==Calculations==

see http://www.ncbi.nlm.nih.gov/pmc/articles/PMC55695 for considerations on calculations

==References (Saltiel Lab)==

915

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