6
edits
Changes
no edit summary
#Wash cells 3x in cold HES (20 mM Hepes, pH 7.4; 1 mM EDTA, 255 mM Sucrose)
#Dry cells well and add 0.6 mL HES (w/Protease Inhibitor) per 150 mm dish
#Scrape cells and homogenize with dounce homogenizer for 20 strokes
