Changes

5 ml {| border="1"! Reagent || per 5mL || per 10 ml mL || Location|- | 40% Methylamine || 1.34 ml mL || 2.68 ml 40% methylaminemL || Solvent Cabinet|- | Methanol || 2.29 ml mL || 4.57 ml methanolmL || Solvent Cabinet|- | n-Butanol || 0.57 ml mL || 1.14 ml nmL || Solvent Cabinet|-Butanol (1-Butanol)0.805 ml | ddH20 || 1.61 ml ddH2O34 mL || 2.68 mL || |- |}

{| border="1"! Reagent || for 10 samples || Location5 ml |- | n-Butanol (1|| 5 mL || Solvent Cabinet|-Butanol)| Ethyl Ether || 1 ml ethyl ether --mL || Solvent Cabinet|-----(this has an expiration date)250 l formic acid ethyl ester| Ethyl Formate || 0.25 mL || Solvent Cabinet|} 

100 ml mL (Enough enough for 8 samples)10 ml {| border="1"! Reagent || Volume || Location|- | [[10X Amino Acid Stock]] || 10 mL || OR Amino Acids –His|-Leu-Trp-Ura 10 ml 10X Potassium Salts10 ml | [[NaCl, MgCl2, CaCl2, Soln Solution]] || 10 ml 10X Ammonium SulfatemL || 1 ml |- | [[100X Vitamin Stock ]] || 1 ml 100X Trace ElementsmL || 5 ml |-| 40% Glucose (|| 5 mL || |-| [[10X Potassium Salts]] || 10 mL |||-| [[10X Ammonium Sulfate]] || 10 mL |||- | [[100X Trace Elements]] || 1 mL |||- | Water || 53 mL|} When adding back amino acids Use: 1080 l uL Uracil (2mg/ml) ;53 ml ddH2O 400 uL Tryptophan (10 mg/ml); 864 uL Leucine ; 216 uL Histidine)