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==Materials==
==Protocol==
#Grow and overnight culture of yeast in the appropriate media.
#Spin cells down and resuspend in FM4-64 for 1h.
#Quench cells for 3h.
#Mount cells on coverslip and analyze by fluorescent microscopy
[[Category:Yeast]]
[[Category:Immunoflouresence]]
==Protocol==
#Grow and overnight culture of yeast in the appropriate media.
#Spin cells down and resuspend in FM4-64 for 1h.
#Quench cells for 3h.
#Mount cells on coverslip and analyze by fluorescent microscopy
[[Category:Yeast]]
[[Category:Immunoflouresence]]
