== Cholesterol Assay ===== Materials ===* Reagent* Cholesterol Standard === Protocol ===* Make 10X stock dilution: Add 50ɥL Cholesterol Standard 450ɥL water to a 1.5mL tube* Pipette 100ɥL of Reagent to 22 wells* Add 2-10ɥL of 10X stock dilution to wells in columns 2-6 in increments of 2ɥL. Each column will have 2 replicates per volume (1 replicate per row). * Repeat in columns 7-11 with 2-10ɥL of Cholesterol Standard (1X dilution) * Allow reaction to proceed for 15 minutes* Read absorbance
