164
edits
Changes
→Analysis/Quantification
#Click '''Redraw Boundaries''' and drab the boundary to include all lanes (not ladder(s)).
#To select the bands of interest, select the '''700 or 800 channel''' by clicking the '''X''' box next to the channel on the right side of the screen (with the vertical '''Display''' tab selected). Add boxes to your bands (see below) and repeat for each channel. Each band of interest should have one box around it.
#Click '''Select''' in the '''Bands''' menu.
#Click the vertical '''Profiles''' tab (below the '''Display''' tab. Adjust the edges of the boxes around each band to include the entire band using the graphs. Each edge should be on the axis and should capture the desired peak(s).
#Once all bands are adjusted, click the '''Western Bands''' tab within the table at the bottom of the screen.
#Click the triangle in the top left corner of the table to copy the table (or the lanes you want). Copy into an Excel spreadsheet or a Text file to use in Excel on your own computer.
#Save your work/'''Work Area''' within '''ImageStudio''' and save pictures (without features) to your flash drive. You can remove the features by unchecking the boxes in the '''Show''' menu bar on the right of the top menu bar.
Note: To quantify the total protein scan, use steps 4-7, but with adjustments to 7.
