Changes

Nr3c1 Genotyping

167 bytes added, 19:00, 17 November 2020

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Make nr3c1 primer dilution at 1uM (10uL each primer + 980uL water) and Cre at .4uM. Primers in '''Genotyping Box''' are marked with a star.

==PCR PROTOCOL==

* 94 °C 2 min

* 10 cycles of:

** 94 °C 20 s

** 65 °C 15 s

** 68 °C 10 s

*28 cycles of:

**94 °C 15 s

**60 °C 15 s

**72 °C 10 s

*72 °C 2 min

[[ Category:Genotyping ]]

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