164
edits
Changes
added band size
*4EBP1- Rev: GAC CTG GAC AGG ACT CAC CGC (2X)
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)
WT= 175bp KO= 550bp
'''4EBP2'''
*4EBP2-Rev: CAG CAC CTG GTC ATA GCC GTG (2X)
*4EBP1/2- Neo Fwd: GCA TCG AGC GAG CAC GTA CTC (1X)
WT= 450bp KO= 750bp
Make primers at 1uM (Fwd1 + Rev1, Neo Fwd1/2 + Rev1, Fwd2 + Rev2, and Neo Fwd1/2 + Rev2). PCR 4EBP1 and 4EBP2 primers separately.
