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Genotyping Program

149 bytes added, 15:21, 5 June 2020
added mc4r-Cone
!| Allele || Program|| Fragment Size(s) || Notes
|-
|[[TSC-floxed Genotyping| TSC-floxed]]|| [[Cre PCR]] || KO- 368bp, WT- 295bp || Two bands if heterozygote
|-
|[[Cre Genotyping|Cre]] || [[Cre PCR ]] || || Only present if Transgene Positive (cannot detect homozygosity at transgene)
|-
|[[TC10b Genotyping|TC10b]] || TC10b || KO - 500bp, WT-1kb || Two bands if heterozygote
|-
|[[Ob/ob_Genotyping|ob/ob Genotyping]] || ob/ob|| KO - 123bp, WT-104kb, control 191 bp || Two reactions, see [[Ob/ob_Genotyping|ob/ob Genotyping]]
|-
|[[MTM1 Genotyping|MTM1]] || MTM1 || Mutant-575575bp, WT-714 714bp || Separate Reactions for WT and KO
|-
|[[CAP Genotyping|CAP]] || CAP || || Two bands if heterozygote, upper band for KO
|[[Vinexin Genotyping|Vinexin]] || Vinexin || ||
|-
|[[nr3c1 Genotyping| GR KO]] || nr3c1 || KO- 300300bp, WT- 247 247bp || Two bands if heterozygote|-|[[mc4r-Cone Genotyping| mc4r-Cone]] || mc4r || KO- 540, WT- 400 || Two bands if heterozygote (run primers separately
|}
 
 
[[ Category:Genotyping ]]
[[ Category:Mouse Work]]
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