==Materials==
#*0.1M NaBicarbonate (0.8401g in 100mL ddH<sub>2</sub>O) and 0.5MNaCl (2.922g in 100mL ddH<sub>2</sub>O)at pH 8.0.#*50mM Sodium Acetate (0.41g in 100mL ddH<sub>2</sub>O)at pH 4.0#*50mM Tris (0.61g in 100mL ddH<sub>2</sub>O) and .5M NaCl (2.922g in 100mL ddH<sub>2</sub>O) at pH 8.0#*0.4M glycine (3.004g in 100mL ddH<sub>2</sub>O)at pH 1.8.
==Procedure==
