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*** 1 mM Pyruvate (100 uL of a 1M stock)
** Re-adjust the pH to 7.4 (7.35-7.45)
* Wash cells twice with 500 mL uL of pre-warmed media, add 500 uL final volume to each well
* Incubate for 1h in non-CO2, humidified, 37C incubator to get rid of any extra CO2
* While plate is incubating, prepare injection solutions in 1.8 mL of prepared XF Media. These are standard concentrations, and should be optimized for your system (especially FCCP)
