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→Experimental protocol
== Background ==
Lipolysis is the hydrolysis of triglycerides into glycerol and free fatty acids. Lipolysis is induced through activation of beta adrenergic receptors. Isoproterenol/Isoprenaline is a non-selective beta adrenergic agonist structurally similar to adrenaline. By administering isoproterenol ''in vivo'', it is possible to artificially stimulate whole-body lipolysis and assess changes in the concentrations of the products of lipolysis (i.e., glycerol and free fatty acids) in the blood.
==Reagents Needed==
(−)-Isoproterenol hydrochloride (Sigma cat# I6504)
PBS
Isofluorane
== Experimental protocol ==