Ppp1r3c Genotyping

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PRIMERS

Fwd: TGCTCTCCTTTTCTCCACGT
Rev: CCGGACCTGAACCTTCTTCT
Neo Rev: GCAGCGCATCGCCTTCTATC

Make primers at 1uM (Fwd + Rev and Fwd + Neo Rev}. PCR primers separately.

PCR PROTOCOL

PCR Step	Temperature	Time	Cycles	Notes
1. Initiation/Melting	95	3:00	1
2. Denaturation	95	0:30	35
3. Annealing	60	1:00	35
4. Elongation	72	1:00	35	steps 2-4 to be run in sequence
5. Amplification	72	5:00	1
6. Finish	4	infinite	n/a