Induction Conditions

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Protein	IPTG	Time	Temperature	Notes
GST-PLC	10 uM	Overnight	28C	very soluble and easy to induce.
GST-HRS	10 uM	Overnight	28C	very soluble and easy to induce.
GST-HRS (2xFYVE)	10 uM	Overnight	28C	very soluble and easy to induce.
GST-Rab5	10 uM	Overnight	28C	very soluble and easy to induce.