TaqMan SNP Assay

From Bridges Lab Protocols
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Materials

  • TaqMan® Genotyping Assay (supplied at 20X, 40X or 80X) (Applied Biosystems).
  • TaqMan Genotyping Master Mix (supplied at 2X)(Applied Biosystems).
  • StepOnePlus Real-Time PCR System (Applied Biosystems).
  • PC/Laptop with StepOnePlus Software installed.
  • MicroAmp Fast 96-Well Plates.
  • Optical Plate Covers.
  • Nuclease-Free Water
  • Sample Genomic DNA (extracted as described Blood DNA extraction)
  • Postive control(s) (Minor allele) (optional, but recommended)


Protocol

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