Tamoxifen Injections for Inducible Knockouts
From Bridges Lab Protocols
- Make up the tamoxifen in corn oil at 10mg/mL (100 mg in 10mL corn oil)
- Inject 40 mg/kg on three consecutive days, at the same time. The time is important, otherwise they will overdose if you inject too close together.
- To determine how much to inject weigh the mouse and inject 4 uL/g of mouse. This means a 25 g mouse will get an injection of 100 uL.
