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| Allele |
Program |
Fragment Size(s) |
Notes
|
| TSC-floxed |
Inoki PCR |
|
Two bands if heterozygote
|
| Cre |
Inoki PCR |
|
Only present if Transgene Positive (cannot detect homozygosity at transgene)
|
| Vac14 |
Inoki PCR |
|
Two bands if heterozygote
|
| TC10b |
TC10b |
KO - 500bp, WT-1kb |
Two bands if heterozygote
|
- Bring up to 500 mL with ddH20
- Sterile filter buffer and store at 4C