Bridges Lab Protocols

SDS-PAGE Separating Gel Buffer

Revision as of 18:56, 3 August 2009 by Davebrid (talk | contribs) (Created page with 'Category:SDS-PAGE Category:Electrophoresis Category:Buffer For 1L: *181.66g Tris *40 mL 10% SDS (or 4 g SDS) *Add water to near 1L *Adjust pH to 8.8 *Bring volume up ...')
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For 1L:

  • 181.66g Tris
  • 40 mL 10% SDS (or 4 g SDS)
  • Add water to near 1L
  • Adjust pH to 8.8
  • Bring volume up to 1L
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