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CHAPS Lysis Buffer (for 10mL lysis buffer)
|
Final Concentration |
per 10 mL |
Stock |
Location
|
| HEPES, pH 7.4 |
40 mM |
400 uL |
1M |
4C
|
| NaCl |
120 mM |
300 uL |
4M |
Shelf
|
| EDTA |
2 mM |
40 uL |
0.5M |
Shelf
|
| CHAPS |
0.3% |
300 uL |
10% |
|
| NaPPi |
10 mM |
400 uL |
0.25M |
Shelf
|
| beta-glycerophosphate |
10 mM |
100 uL |
1M |
4C
|
| NaF |
50 mM |
1mL |
0.5M |
Shelf
|
| Protease Inhibitors |
|
1 mini tablet |
|
4C
|