Bridges Lab Protocols

50X TAE Buffer

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Reagents

  • 900mL ddH2O
  • 242g Tris Base
  • 57.1 mL Glacial Acetic Acid
  • 18.6g EDTA

1. Add Tris Base, Glacial Acetic Acid, and EDTA to 500mL water in 1L Erlenmeyer Flask and mix well by swirling the flask. 2. Add remaining 400mL water and mix on stir plate (or my swirling).

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