Preparing and Sending Tissues to Histology Core

1. Harvest tissue from animal, place into cassette and submerge in 10% neutral buffered formalin for 24-48 hrs (depending on thickness of sample).
2. Move cassettes into 70% ethanol for 24 hrs. Samples can be left in 70% for long term storage, if required. Make sure the storage container is sealed so that the ethanol does not evaporate and samples are submerged for the entire storage period.
3. Bring cassettes in a biohazard bag (with 70% EtOH) and completed request form (place in pocket of bag) to the UMCCC Tissue Core, information on location, phone number and hours of operation can be found here: https://www.pathology.med.umich.edu/giordano_lab/histology.htm. The form can be found here
4. Cassettes, bags and order request forms (must be copied, no link online) can be found in the lab in the large cabinet to the left of the sink.