Dave must add you to the lab under his account, he will give you your username and the password needed
Typically we will select Sanger Sequencing (the only low throughput option)
Select 'Submit samples/plates'
Select the appropriate short-code from the drop down menu at the top of this page
Generally choose 'One-at-a-time Sample Submission' unless you have a lot
From here enter the following for each section:
The sample name (the sample name that mean something to you regarding the sequence)
No discounts
Mini-Prep Plasmid
Whatever vector you used for this plasmid
Size of the insert you are sequencing
Custom Primers
Do not save
Do not rush
Save and repeat with your other samples
Once you have finished adding your samples ID codes for each sample will be generated and the last four digits of this ID are what you need to use for the tubes you are submitting, not the sample names you made
Samples should be submitted in 1.5mL tubes
Make sure to write these IDs very clearly
A submission ID will also be generated and you need to know this when you drop your samples off so they can find your sequence request in their system
Plasmids need to be diluted to a concentration of ~50ng/ul and total volume should be 10ul and should be labeled with the generated sample ID
In addition to your samples you will need to provide an aliquot of the primer
Volume is 10ul/sample you submit
Should be labeled as the range of sample IDs generated and P1 (example: xxxx-xxxxP1), if you have more than one primer write xxxx-xxxxP2 and so on