*Mouse Tissues (Frozen)
#Add a stainless steel bead and keep tissues on ice. Homogenize using Qiagen Tissue Lyser (3 min at 25Hz for WAT/Liver
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1 KB (253 words) - 16:06, 25 April 2019
*When collecting tissues, keep tissues in 10% formalin solution for ~24 hours at 4 degree C.
*Transfer tissues to PBS after for long-term storage at 4 degree C.
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4 KB (624 words) - 17:05, 8 June 2020
[[Category:Tissues]]
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882 bytes (140 words) - 21:43, 5 January 2018
[[Category:Mouse Tissues]]
...uL digested glycogen for fasted samples, and use a 10X dilution for refed tissues (using either 5 or 2ul from the diluted sample)
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2 KB (376 words) - 13:02, 19 June 2017
*[[Triglyceride Assay from Cells and Tissues]]
*[[Preparation of Protein Lysates from Mouse Tissues]]
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4 KB (484 words) - 12:10, 15 August 2016
...on as you normally would for RNA extraction, ie by mechanically disrupting tissues in Qialyser. Approximately 20 mg of tissue is sufficient.
[[ Category: Mouse Tissues ]]
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3 KB (417 words) - 17:42, 25 September 2015
[[ Category: Mouse Tissues ]]
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1 KB (173 words) - 14:28, 2 August 2017
# Divide this by two for diploid cells/tissues to get the number of mitochondria per cell
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1 KB (201 words) - 17:27, 4 April 2023
...y an intraperitoneal injection of insulin. As glucose is transported into tissues and glucose production is suppressed, glucose levels drop. A more insulin
Since most glucose is taken up into non-fat tissues, it can be confounding if you dose based on total body weight, and the two
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4 KB (725 words) - 16:43, 10 February 2025
# Prepare samples in 1 mL TRIzol either from cells or from tissues lysed in the homogenizer. These lysates can be stored indefinitely at -80.
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2 KB (280 words) - 21:35, 29 December 2014
[[Category:Tissues]]
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3 KB (478 words) - 20:51, 22 October 2018
...ly 3-10% formalin or paraformaldehyde solutions are used to perfuse or fix tissues.
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9 KB (1,296 words) - 13:17, 6 June 2022