* Aliquot to 50 uL in tissue culture freezer
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===Cell and Tissue Culture===
*[[Generating DMSO Stocks for Cell Culture]]
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...ia'''. Filter together Media (500 mL) and Pen/Strep (10 mL) into a tissue culture bottle
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# Turn radioactive tissue culture incubator on at 37C, make sure to turn on the CO2 as well.
# Place in the radioactive tissue culture incubator for 60-120 minutes.
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4. 35 mm cell culture dishes (or 6-well plates)
#Place into culture dish containing 2 mL of incubation medium & allow to recover in incubator (
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....2 of [https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3523695/ Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells] and the
...h the tendons so as to not nick the muscle, snip the underlying connective tissue, effectively removing the skin.
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## Calculate glycogen levels as umoles glucose released per g tissue (should be ~10)
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# Turn heating block up to 100C. Turn radioactive tissue culture incubator on at 37C, make sure to turn on the CO2 as well.
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...are thawed (PP00000160) at least two weeks prior to end of the three-month culture period.
...esion during routine culture, the RAW 264.7 cells are grown on sterile non-tissue–grade plastic (ultra-dish Petri dishes). Adherence of macrophages to these
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