*Decide on flow rate and operating pressure (see [[HPLC - Deciding on a Flow Rate and Pressure Settings]])
*Select '''HPLC stack'''
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==HPLC Setup==
...k that the HPLC is set up for protein work and not radioactive work (see [[HPLC - Plumbing Setup]]).
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*To connect or disconnect a column or sample loop see [[HPLC - Plumbing Setup]]
*Pick your Method (see [[HPLC - Designing a Method or Sequence ]])
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## Place the samples at -20C until loading on HPLC (Be sure to clean Speed Vac)
===HPLC Sample Preparation===
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** purification: anion exchange (AX) HPLC
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