* This protocol is for using a pre-ranked gene list
* You should have a list of all analysed genes, sorted be gene expression changes (log fold change or fold change) from highest differentially expres
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3 KB (434 words) - 13:31, 5 September 2018
...to the lumi data frame. Phenotype data can be accessed by pData(lumi) and expression data can be accessed by exprs(lumi).
==Differential Expression Analysis==
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5 KB (722 words) - 15:34, 2 September 2009
...the BSData dataframe. Phenotype data can be accessed by pData(BSData) and expression data can be accessed by exprs(BSData).
==Differential Expression Analysis==
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5 KB (766 words) - 15:34, 2 September 2009
#Choose target vector and region of interest in gene
...for eukaryotic expression: GCCACCATGG) or Shine-Dalgarno (for prokaryotic expression) sequence between the 5' restriction site and the complementary sequence (b
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2 KB (247 words) - 20:19, 5 May 2009
# Input: expression matrix, chip environments
# output: heatmaps of all the pathways for all the genes in expression matrix
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3 KB (406 words) - 15:35, 2 September 2009
[[ Category: Gene Expression ]]
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1 KB (234 words) - 18:52, 30 December 2014
• Taqman Gene Expression Assay Primers stored at -20
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2 KB (302 words) - 15:16, 10 May 2017
* Mature miRNA Primer, this is a gene specific primer corresponding to the mature miRNA sequence.
* The SYBR Green-based real-time PCR was performed to quantify miRNA expression, and U6 can be used for normalization.
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3 KB (432 words) - 15:24, 1 March 2017
...0uM stock is prepared by adding 227 uL of distilled water to 22.7nmol of a gene as an example- this is 4uL Forward Primer, 4 uL Reverse Primer and 992 uL W
[[ Category: Expression ]]
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4 KB (585 words) - 19:13, 8 November 2023