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Chromatin Immunoprecipitation

78 bytes added, 15:23, 20 January 2016
→‎Buffers and Solutions Needed: made subsections
Johnson DS, Mortazavi A, Myers RM, Wold B. Genome-Wide Mapping of in Vivo Protein-DNA Interactions. Science (80- ) 316: 1497–1502, 2007. [http://dx.doi.org/10.1126/science.1141319 doi:10.1126/science.1141319]
===Buffers and Solutions Needed===
* 20% Formaldehyde (from 37% formaldehyde Sigma F87750)
* 2.5M Glycine
* TE: 10 mM Tris 7.5, 0.1 mM EDTA (cold)
* [[ChIP Elution Buffer]]
 
===Equipment===
* Cool microfuge and swinging bucket centrifuge down to 4C