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Assessing isoproterenol-stimulated whole-body lipolysis in vivo

124 bytes added, 15:21, 10 March 2015
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→‎Experimental protocol
#Inject 10 mg/kg isoproterenol (prepared fresh in sterile PBS) via intraperitoneal injection.
#Wait 15 minutes.
#Briefly anesthetize mice with isoflurane and collect blood via retro orbital bleed. Allow blood to clot over ice.***Note: Take care when anesthetizing mice the second time. They will go under VERY quickly, so watch the mouse closely***
#Centrifuge blood samples and collect serum.
#Assay serum for NEFA's and Glycerol/Triglycerides using Wako and Sigma assay kits, respectively.