Changes

*Weight Weigh out 2.08g of each Tween 80 and PEG into a 50 mL falcon tube. Bring up to 40 mL with water and filter by steriflip. This is 5.2% PEG/Tween*Dissolve rapamycin at 20 mg/mL (0.0219 M) in waterethanol (prepare as a 1 mL aliquot and store at -20 C).*Add 25 uL of 20 mg/mL RAPA in 5 mL Tween 80/PEG diluent to get a 0.1 mg/mL concentration (109 uM; use this for 1 week).*Inject IP inject 10 uL/g body weight(1 mg/kg)