Splitting Cells: Difference between revisions

changed serum names
added temperature
Line 12: Line 12:
#Add 9 mL media to trypsinized cells
#Add 9 mL media to trypsinized cells
#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
#Add 1 mL cells to each dish (for 10X dilution; 0.5 mL for 20X)
#Replace plates in the incubator
#Replace plates in the 37C incubator


==Cell Specific Notes==
==Cell Specific Notes==