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Created page with " == Protocol == #After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N #After fixation, begin d..."
== Protocol ==
#After harvesting tissue from animal, place into pathology cassette and fix in 4% formaldehyde, 4% paraformaldehyde or 4% formalin O/N
#After fixation, begin dehydrating the tissue in 70% Ethanol
#On the day of planned embedding, wash sample with 100% Ethanol 3 times for 30 minutes.
##While washing in 100% Ethanol, start the paraffin wax machine (Link Building, Room 311)
#After 100% Ethanol washes, Wash 3 times in Xylenes
#Wash samples in a beaker filled with wax at 58-60C, 3 times for 15 mins
#Immediately before embedding, spray wax mold with "Mold Grease" and coat the bottom layer of the mold with wax
#Take your sample and orient it on top of the base wax as desired
#Place pathology cassette on top of the mold and fill with wax covering the mold
#Place mold with sample on "Cold Side" of embedding station
##Wax will solidify in 10-15 minutes
#Remove wax embedded sample from the mold (should just slip out)
#Store at room temperature until prepped to section