915
edits
Changes
specified insulin concentration
*Radioactive <sup>14</sup>C-2-deoxyglucose
*Hot 2-DG. Need 50 uL per well, prepare 1 mL KRBH/BSA, 1 uL cold 2-DG and 50 uL radioactive 2-DG. count 3X5 ul of hot 2DG.
*Insulin stock solution (1 mg/mL)
==Protocol==
#Starve cells >3h in 0.5% FBS
#Prepare insulin in KRBH/BSA (0.6uL (100nM) insulin/mL), needing 0.5 mL per well)
#Wash cells 2x with warm PBS -/-
#Add 450 uL KRBH/BSA with or without insulin to wells. Typically do triplicate measurements