Open main menu

Changes

PCR Amplification of DNA

11 bytes added, 13:24, 5 May 2009
m
→‎Protocol
*Run PCR Program. Normally use touchdown PCR ('''DAVETD''') as follows:
:#1 min at 94:#30s at 65:#2 min/kb at 72:#30s at 94:#30s at 63 then -0.5/cycle:#2 min/kb at 72:#Repeat steps 4-6 28 times:#30s at 94:#30s at 45:#11 min at 72:#hold Hold at 4 until ready
*Purify PCR product if necessary using Qiagen kit (Add 5x PB)