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##1 min at 94##30s at 65##2 min/kb at 72##30s at 94##30s at 63 then -0.5/cycle##2 min/kb at 72##Repeat steps 4-6 28 times##30s at 94##30s at 45##11 min at 72##hold at 4 until ready
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*Run PCR Program. Normally use touchdown PCR ('''DAVETD''') as follows:
*Purify PCR product if necessary using Qiagen kit (Add 5x PB)
