915
edits
Changes
m
##1 min at 94##30s at 65##2 min/kb at 72##30s at 94##30s at 63 then -0.5/cycle##2 min/kb at 72##Repeat steps 4-6 28 times##30s at 94##30s at 45##11 min at 72##hold at 4 until ready
no edit summary
*Run PCR Program. Normally use touchdown PCR ('''DAVETD''') as follows:
*Purify PCR product if necessary using Qiagen kit (Add 5x PB)
