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#Incubate in 500X secondary solution 45 minutes in 2% BSA PBS
#Wash coverslips 3 times 10 minutes with PBS with gentle rocking
#Add 10 uL vectashield to glass slide and place cells on slide(coverslip side up, use sharp tweezers and tip, 2 coverslips on each slide). Label slide with date, cells and treatment + color code for antibodies. Fix with nail polish, dry for at least 1 hourup to ON in dark drawer. Store long term in sealed box at 4C.
*It is possible to use ImageJ to analyse [[Colocalization]]
[[Category:Immunofluoresence]]