Changes

Cesium Chloride Preparation of DNA

3 bytes added, 22:03, 17 January 2012

updated cesium concentration

#Load into two ultracentrifuge tubes then add 25 uL EtBR to each tube

#Centrifuge 60 000 RPM (250 000g) O/N in NVT90 rotor

#Withdraw the upper band with a 18g needle and reload into 0.1g/L mL CsCl for second spin

#Remove band and extract EtBr with butanol until clear

#Dialyse overnight in 2L of TE

#Precipitate with 3 vol absolute ethanol and 1/10 vol of 3M Acetate pH 7

#Resuspend to a final concentration of 5-10 mg/mL in TE

915

edits