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Glycogen Determination from Tissues

40 bytes added, 16:42, 13 January 2012
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described preparation and storage of amyloglucosidase
* Ethanol
* 50 mM Sodium Acetate, pH 4.8
* Amyloglucosidase 0.3 mg/mLin 50 mM Sodium Acetate. Stored in -80
* Glucose quantification kit (Wako Autokit Glucose Buffer Solution cat # 439-9091; for protocol see http://www.wakodiagnostics.com/pi/pi_autokit_glucose.pdf)
* Glucose standard solution (200 or 500 mg/dL; Wako)